مطالعه تاثیرگذاری هورمون محرک فولیکولی (FSH) در حضور سرم های مختلف حیوانی روی رشد فولیکولی در محیط In vitro

Backgroundovarian follicle growth and oocyte maturation. In vitro follicular culture systems, at variousdevelopmental stages, allow the identification of these factors and the understanding of theirmechanisms of action. Keeping in mind the significance of the influence of environmental factors onthe follicle growth, this work focuses on the effect of some endocrine and paracrine factors on thegrowth and differentiation of preantral follicles during the in vitro follicular culture using the rodent(mouse) model.: Several endocrine and locally acting factors are involved in the complex process ofMethodsserum type. Prepubertal gilt serum, embryonic stem cell tested fetal calf serum, hypogonadal mouseserum and fetal calf serum were tested during the present study. Effect of the culture period was alsoevaluated on the follicle growth. After carefully selecting the optimum growth conditions, the effectof FSH was evaluated on the growth and viability of the follicular and oocyte maturation. Differentconcentrations of FSH (5, 20, 40, 60, 100, 140, 180 and 220 mIU/l) were added to the culture medium(containing 25-30 follicles each) during separate experiments.: Our study was semi-experimental. First task of the study was to choose an appropriateResultsDuring the present experiment, 100 mIU/ml FSH showed highly significant effect on the follicle andoocyte growth. Follicle survival rate also increased (91%) as compared to that grown without thisgonadotropin (28%). Oocyte maturation (61%) and germinal vesicle breakdown rates (81%) alsoshowed an increase (p: After experiments, fetal calf serum (FCS) was chosen for the evaluation of the effect of FSH.≤0.05).Conclusionantrum had a positive effect on the follicle survival and oocyte robustness.: These results have suggested that the exposure to FSH and FCS before the formation of